Journal: Microorganisms

Article Title: Deciphering Antibody Responses to Orthonairoviruses in Ruminants

doi: 10.3390/microorganisms9071493

Figure Lengend Snippet: Serological assays.

Article Snippet: Furthermore, all NSDV antisera were run in three different CCHFV ELISA systems and one indirect immunofluorescence test.

Techniques: Indirect ELISA, Enzyme-linked Immunosorbent Assay, Fluorescence, Control

Journal: Microorganisms

Article Title: Deciphering Antibody Responses to Orthonairoviruses in Ruminants

doi: 10.3390/microorganisms9071493

Figure Lengend Snippet: Euroimmun iIFA with sheep NSDV antisera: The results obtained with the serum of an infected sheep (P2) are presented as an example together with the results for the control sheep (O2). A specific staining is demonstrated for cells expressing CCHFV GPC ( B ) as well as CCHFV N ( D ) for P2. All controls (cell control: ( E ); serum prior to infection: ( A , C )) did not lead to a positive signal. The control sheep O2 did not test positive in any condition ( F – J ).

Article Snippet: Furthermore, all NSDV antisera were run in three different CCHFV ELISA systems and one indirect immunofluorescence test.

Techniques: Infection, Control, Staining, Expressing

Journal: Microorganisms

Article Title: Deciphering Antibody Responses to Orthonairoviruses in Ruminants

doi: 10.3390/microorganisms9071493

Figure Lengend Snippet: Euroimmun iIFA with cattle NSDV antisera: Results for one infected calf (Q6) are exemplarily presented together with the results for the mock control calf (S2). A specific staining is demonstrated for cells expressing CCHFV GPC ( B ). All controls (cell control: ( C ); serum prior infection: ( A ) did not lead to a positive signal. The control calf S2 did not test positive under any circumstances ( D – F ).

Article Snippet: Furthermore, all NSDV antisera were run in three different CCHFV ELISA systems and one indirect immunofluorescence test.

Techniques: Infection, Control, Staining, Expressing

Journal: Microorganisms

Article Title: Deciphering Antibody Responses to Orthonairoviruses in Ruminants

doi: 10.3390/microorganisms9071493

Figure Lengend Snippet: Specificity and sensitivity of HAZV, DUGV, and NSDV assays: Panel ( A ) shows the proportion of positive tested sera within a specific assay in relation to all sera following infections/immunizations with the same challenge virus, which were at least positive in one homologous virus-specific test. Exemplarily, seven sera from the DUGV studies were tested positive in the NSDV PRNT out of ten animals with DUGV antibodies detectable via any DUGV assay, leading to a bar of approx. 70% length. Panel ( B ) visualizes the ranking order of sensitivity and specificity for all diagnostic assays, with highest sensitivity achieved by PRNT and highest specificity by ELISA.

Article Snippet: Furthermore, all NSDV antisera were run in three different CCHFV ELISA systems and one indirect immunofluorescence test.

Techniques: Virus, Diagnostic Assay, Enzyme-linked Immunosorbent Assay